I am a Pharmacist, I got a PhD in Analytical Chemistry and I currently work as a postdoc at the group of Prof. S. Rudaz in the Pharmaceutical Analytical Chemistry lab, University of Geneva.
My current research focuses on a new approach to interface capillary electrophoresis with mass spectrometry detection, with the aim to push many of the limitations of this versatile hyphenatied technique apart.
I developed my PhD work in the group Heterocycles of Biological Interest, led by Prof. J. Carlos Menéndez and Prof. M. A. Martín. My previous background comprises the optimization of liquid chromatography methods, fundamental studies on HPLC separations, and the development of analytical strategies to study topoisomerase I inhibitors derived from luotonin A, keeping in mind the minimization of environmental impact of the techniques.
I am used to work on liquid chromatography and on fluorescence spectroscopy techniques. I have also worked on the development of enzimatic and cell-culture assays.
- Use of cyclodextrins as mobile phase additives on HPLC to achieve an environmental friendly chromatography method for analysis β-carboline alkaloids on C18 columns. By using C1 stationary phases and SPE, we were able to develop an even greener alternative.
- My group has developed and fully validated a simple HPLC method to analyze six luotonin A analogues in spiked human serum samples.
- We employed fluorescence spectrometry to study the fluorescence properties of luotonin A analogues, particullary how the solvent nature and the pH affected the fluorescence, and also how some of these derivatives bind to DNA.
- I have also worked on how cyclodextrins can improve the solubility of luotonin A derivatives to enhance their pharmacological properties, developing an environmentally respectful, miniaturized fluorescence assay for solubilization studies. We also elucidated the nature of the drug-cyclodextrin complexes by using ESI-GC.
- We have stablished the citotoxicity of these alkaloids by using topoisomerase I inhibition and cell growing inhibition assays, and elucidated a new binding mode to their target complex by means of molecular modelling techniques.
- On the HPLC field, we have carried out a physicochemical comparison of the performance of fully porous 5 μm silica particles vs. 2.6 μm core-shell silica particles for C18, pentafluorophenyl and phenyl-hexyl chemistries. We have also fully validated a methodology to quantify the luotonin A derivatives on cell cultures by using this new column technology.
- I have participated in a review on analytical methods for NSAIDs, a book charpter on how drug-DNA interactions can be investigated, and a review on core-shell particles technology applied to HPLC.
- As side-lines, I have also worked on the development of a carbazole-based fluorescent probe for anions, fluorescent sensors for radicals and fluorescent drugs as theranostic agents to detect and treat protein misfolding diseases.
- I have teached Analytical Chemistry and Instrumental Analysis practical classes for six years, and as a result I have recently published a paper on The Journal of Chemical Education, journal for which I act as a reviewer too. I have also participated on a Teaching Innovation project on analytical chemistry and been co-advisor for two students' Final Degree Projects.